abstract：:The sequential changes in the histology in scabietic lesions were investigated for hosts (rabbits) during sensitization and reinfestation with Sarcoptes scabiei. Experimentally infested naive hosts developed heavy scabies infestations but 65% of the hosts showed induced immunity (resistance) to reinfestation. All host...

journal_title：Experimental parasitology

authors： Arlian LG,Rapp CM,Vyszenski-Moher DL,Morgan MS

更新日期：1994-02-01 00:00:00

abstract：:Polymerase chain reaction amplification of several polymorphic genes has been used to study the population biology of Plasmodium falciparum. S antigen is particularly suitable for such studies, but difficulties in the amplification of this gene have precluded its use. Here we describe a simple method for the amplifica...

journal_title：Experimental parasitology

authors： Kyes S,Craig AG,Marsh K,Newbold CI

更新日期：1993-12-01 00:00:00

abstract：:Snails from a B. glabrata stock, selected for nonsusceptibility to the NMRI strain of S. mansoni, exhibited unusual schistosome infections, with multiple sporocysts prominent in the head-foot (foot-sporocysts). When F1 progeny from numerous crosses between well-defined susceptible (S) and resistant (R) parental snails...

journal_title：Experimental parasitology

authors： Lewis FA,Richards CS,Knight M,Cooper LA,Clark B

更新日期：1993-11-01 00:00:00

abstract：:Mitochondria from a rodent malarial parasite (Plasmodium berghei) were successfully purified by differential centrifugation and 22% Percoll density gradient separation. The purified mitochondria from the erythrocytic stages of the parasite had a density of 1.05 and were found to be heterogeneous by transmission electr...

journal_title：Experimental parasitology

authors： Krungkrai J,Krungkrai SR,Bhumiratana A

更新日期：1993-09-01 00:00:00

abstract：:Mice infected with Plasmodium berghei K173-parasitized erythrocytes develop severe hypothermia followed by death as a consequence of murine cerebral malaria early in the second week after infection. A single intraperitoneal injection of 10(5) Units of IFN-gamma given between Day 4 and Day 6 postinfection results in a ...

journal_title：Experimental parasitology

authors： Curfs JH,van der Meide PH,Billiau A,Meuwissen JH,Eling WM

更新日期：1993-09-01 00:00:00

abstract：:To analyze whether nitric oxide may be involved in the pathogenesis of the mouse cerebral malaria (CM), nitrate and nitrite were first measured in urines of Plasmodium species infected mice. The CM-susceptible CBA/J mice were infected with either Plasmodium berghei or Plasmodium chabaudi, and the CM-resistant BALB/c m...

journal_title：Experimental parasitology

authors： Asensio VC,Oshima H,Falanga PB

更新日期：1993-08-01 00:00:00

abstract：:How the avermectins cause the elimination of gastrointestinal nematodes from host animals has not yet been clearly identified. Using visual and radiometric parameters to measure oral ingestion in Haemonchus contortus, we showed that ivermectin (IVM) rapidly inhibited ingestion at concentrations > or = 10(-10) M. Motil...

journal_title：Experimental parasitology

authors： Geary TG,Sims SM,Thomas EM,Vanover L,Davis JP,Winterrowd CA,Klein RD,Ho NF,Thompson DP

更新日期：1993-08-01 00:00:00

abstract：:The sporogonic cycle of Plasmodium falciparum was obtained in vitro. Mature gametocytes, from blood-stage cultures, produced gametes that underwent fertilization at elevated pH and ambient temperatures. Wheat germ agglutinin stimulated transformation of zygotes into retorts and ookinetes. Twenty-four hours thereafter,...

journal_title：Experimental parasitology

authors： Warburg A,Schneider I

更新日期：1993-03-01 00:00:00

abstract：:The oocysts of Eimeria tenella, one of the most pathogenic of several species causing chicken coccidiosis, are difficult to distinguish microscopically from several other infective Eimeria species. One copy of a gene coding for 5S ribosomal RNA has been cloned from E. tenella and sequenced. A coding region of 120 nucl...

journal_title：Experimental parasitology

authors： Stucki U,Braun R,Roditi I

更新日期：1993-02-01 00:00:00

abstract：:Giardia lamblia consist of heterogeneous isolates that can be divided into at least three groups. Differential screening of a cDNA library with isolate-specific antisera identified a gene which is expressed and found only in Group 3 isolates. This gene, GLORF-C4, is 597 bp in length and predicts a deduced protein of 1...

journal_title：Experimental parasitology

authors： Nash TE,Mowatt MR

更新日期：1992-12-01 00:00:00

abstract：:Complement activation by protoscoleces of Echinococcus granulosus was studied by analyzing the damage to their tegumental membrane produced by incubation in both normal and hydatid human sera. The state of the apical tegumental membrane was evaluated by measuring the electric potential difference with microelectrodes....

journal_title：Experimental parasitology

authors： Ferreira AM,Trecu T,Reisin I

更新日期：1992-11-01 00:00:00

abstract：:Hemocytes derived from a strain (13-16-R1) of Biomphalaria glabrata resistant to Schistosoma mansoni were significantly more likely to bind untreated latex beads than hemocytes from the schistosome-susceptible M line strain. Beads preincubated in 13-16-R1 plasma were more readily bound by both 13-16-R1 and M line hemo...

journal_title：Experimental parasitology

authors： Uchikawa R,Loker ES

更新日期：1992-09-01 00:00:00

abstract：:Ninety-one Chilean, 15 Bolivian, and 9 Argentinian Trypanosoma cruzi stocks, isolated from various hosts and vectors, were characterized by schizodeme analysis with EcoRI and MspI endonucleases. The three major similar pattern groups that emerged from this sample correlated with results of isoenzyme analysis. This res...

journal_title：Experimental parasitology

authors： Solari A,Muñoz S,Venegas J,Wallace A,Aguilera X,Apt W,Brenière SF,Tibayrenc M

更新日期：1992-09-01 00:00:00

abstract：:Monoclonal antibodies against infective third-stage larvae (L3) of Brugia pahangi were generated from mice immunized with L3 antigens. The monoclonal antibodies were L3 stage-specific or stage-nonspecific. A BpG1 monoclonal antibody (IgG1 subclass) showing L3 stage-specificity was examined in detail. BpG1 recognized t...

journal_title：Experimental parasitology

authors： Oikawa Y,Ikeda T,Horii Y,Fujita K,Tsukidate S

更新日期：1992-08-01 00:00:00

abstract：:We have studied inflammatory reactions in the mesenteric tissue of mice infected with Schistosoma mansoni. Perivascular tissue contained diffuse infiltrates of macrophages, eosinophilic granulocytes and lymphocytes. Angiogenesis in the perivascular adipose tissue was associated with superficial plasmacytogenic foci. P...

journal_title：Experimental parasitology

authors： Weinberg DF,Baldo-Correa E,Lenzi HL,Borojevic R

更新日期：1992-06-01 00:00:00

abstract：:Nine of 18 chimpanzees inoculated with 250 infective third-stage larvae (L3) each developed patent (i.e., positive for microfilariae) Onchocerca volvulus infection. Four of 6 infected chimpanzees that received 200 micrograms/kg ivermectin at 28 days postinfection (pi) became patent, whereas, when ivermectin was given ...

journal_title：Experimental parasitology

authors： Soboslay PT,Weiss N,Dreweck CM,Taylor HR,Brotman B,Schulz-Key H,Greene BM

更新日期：1992-06-01 00:00:00

abstract：:A ceramide-anchored glycophosphosphingolipid antigen was isolated from the lipid extract of Leishmania donovani promastigotes. The affinity-purified glycolipid antigen contained galactose, mannose, myo-inositol, phosphate, ceramide, and hexosamine but no sialic acid. The phosphate group was present internally at the c...

journal_title：Experimental parasitology

authors： De-Majumdar T

更新日期：1992-05-01 00:00:00

abstract：:Among the wide variety of clinicopathological manifestations of intestinal amebiasis, amebomas occur rarely and their pathogenesis is not well understood. When cholesterol-fed, 2- to 4-week-old guinea pigs were infected intracecally with a virulent, monoaxenic strain of Entamoeba histolytica, gross and histologically ...

journal_title：Experimental parasitology

authors： Bhatti HS,Bhushnurmath S,Mahajan RC,Ganguly NK,Sehgal R

更新日期：1992-05-01 00:00:00

abstract：:The biochemical properties of the enzyme responsible for nematode "activated L-serine sulphydrase" activity (L-cysteine + R-SH----cysteine thioether + H2S) have been investigated using primarily the gastro-intestinal nematodes Nippostrongylus brasiliensis and Haemonchus contortus. The activated L-serine sulphydrase en...

journal_title：Experimental parasitology

authors： Walker J,Barrett J

更新日期：1992-03-01 00:00:00

abstract：:The oxidative metabolism of peritoneal macrophages (PM) and neutrophils from nifedipine (calcium channel blocker)-treated, Plasmodium berghei (NK 65)-infected and normal infected Swiss Albino mice was studied. A significant fall in oxidative metabolism as evidenced by decreased chemiluminescence (CL) response (P less ...

journal_title：Experimental parasitology

authors： Kalra A,Dubey ML,Ganguly NK,Mohan K,Mahajan RC

更新日期：1992-02-01 00:00:00

abstract：:Living Leishmania amazonensis amastigotes were incubated with radioiodinated N-benzyloxycarbonyl-L-tyrosyl-L-alanyl diazomethane (Z-Tyr-AlaCHN2), an irreversible inhibitor of mammalian cathepsins B and L. Parasite lysates were subjected to electrophoresis in gelatin-containing sodium dodecyl sulfate-acrylamide gels to...

journal_title：Experimental parasitology

authors： Alfieri SC,Pral EM,Shaw E,Ramazeilles C,Rabinovitch M

更新日期：1991-11-01 00:00:00

abstract：:The opossum Didelphis marsupialis is known to be among the most important wild reservoirs of Trypanosoma cruzi and one in which the trypanosome may go through both the usual vertebrate intracellular cycle in its tissues and an extracellular cycle in the lumen of its scent glands. The species is highly resistant to hea...

journal_title：Experimental parasitology

authors： Jansen AM,Leon L,Machado GM,da Silva MH,Souza-Leão SM,Deane MP

更新日期：1991-10-01 00:00:00

abstract：:Cytoadherence of Plasmodium falciparum-infected erythrocytes to amelanotic melanoma cells was pH dependent; increased adherence was observed in the pH range of 6.1 to 6.8 and was greatest between pH 6.6 and 6.8 Ca2+ promoted cytoadherence, but at higher concentrations (40-50 mM) than is usually the case for cell-cell ...

journal_title：Experimental parasitology

authors： Crandall I,Smith H,Sherman IW

更新日期：1991-10-01 00:00:00

abstract：:Infectivity of the multicellular pathogen Schistosoma mansoni for the human host is dependent upon the ability of free-living cercariae to transform rapidly into parasitic schistosomula. The biochemical pathways that regulate this transitional period are unknown. The role of protein phosphorylation was investigated by...

journal_title：Experimental parasitology

authors： Wiest PM,Olds GR,Bowen WD

更新日期：1991-08-01 00:00:00

abstract：:Spleen cells from BALB/c mice were fused with myeloma cells following infection of the mice with Trichinella spiralis larvae and an ip booster injection with larval homogenate antigen. A monoclonal antibody (Mab), designated as TS 3G6 which did not react with sera or tissue extracts from noninfected mice, rats, and gu...

journal_title：Experimental parasitology

authors： Kehayov IR,Kyurkchiev SD,Tankov CV,Komandarev SK

更新日期：1991-08-01 00:00:00

abstract：:Investigation of protease activities during the transformation of Trypanosoma cruzi epimastigotes into metacyclic trypomastigoes (metacyclo-genesis) revealed three major components with apparent molecular weights of 65, 52, and 40 kDa. The 65-kDa protease is a metacyclic trypomastigote stage-specific protease with an ...

journal_title：Experimental parasitology

authors： Bonaldo MC,d'Escoffier LN,Salles JM,Goldenberg S

更新日期：1991-07-01 00:00:00

abstract：:Immunity to T. cruzi is complex, involving among other components, antibody production, CD4+ helper cells, CD8+ T cells as both regulators and effectors of immunity, and possibly, double-negative T cells. In addition, several of these components have been implicated in pathogenesis in the chronic infection. Although t...

journal_title：Experimental parasitology

authors： Tarleton RL

更新日期：1991-07-01 00:00:00

abstract：:The possible involvement of protein kinase C and Ca2+ metabolism in the proteolytic enzyme release from schistosome cercariae was studied. Cercariae were placed in dechlorinated tap water containing 0.37 mM calcium in the small glass petri dish and exposed to the stimuli (linoleic acid, phorbol esters, and Ca2+ ionoph...

journal_title：Experimental parasitology

authors： Matsumura K,Mitsui Y,Sato K,Sakamoto M,Aoki Y

更新日期：1991-04-01 00:00:00

abstract：:The concentration of L-hydroxyproline in the liver of ICR female mice increased rapidly during the 8th to 11th weeks of Schistosoma mansoni infection. Free L-proline concentration began to increase about the 7th week and reached its maximum at the 8th to 9th weeks of the infection, when the granulomatous response to t...

journal_title：Experimental parasitology

authors： Tanabe M,Kaneko N,Takeuchi T

更新日期：1991-02-01 00:00:00

abstract：:The analysis of PvuII restriction patterns of Leishmania spp. and Trypanosoma spp. genomic DNA showed genus distinctive profiles. A specific PvuII site was detected in the 5' domain of 18S ribosomal DNA of Leishmania. A 20-mer oligonucleotide encompassing this PvuII region was synthesized. This sequence, when utilized...

journal_title：Experimental parasitology

authors： Uliana SR,Affonso MH,Camargo EP,Floeter-Winter LM

更新日期：1991-02-01 00:00:00

abstract：:Immunoelectron microscopy demonstrated the presence of Pf155/RESA in dense granules of Plasmodium falciparum merozoites rather than in micronemes as previously suggested. Since the dense granules are released after the merozoite enters the parasitophorous vacuole, the role of Pf155/RESA in invasion and subsequent step...

journal_title：Experimental parasitology

authors： Aikawa M,Torii M,Sjölander A,Berzins K,Perlmann P,Miller LH

更新日期：1990-10-01 00:00:00

abstract：:A method is described for the isolation of peroxisomes from mitochondria-enriched fractions obtained from both species of nematodes. The distributions of these organelles are characterized after density gradient centrifugation in sucrose or Percoll by urate oxidase and catalase activities. The possession of peroxisome...

journal_title：Experimental parasitology

authors： Paget TA,Fry M,Lloyd D

更新日期：1990-08-01 00:00:00

abstract：:Since we had previously demonstrated the protective role played by Toxoplasma excreted-secreted antigens, the aim of the present work was to produce monoclonal antibodies directed against these antigens in order to determine if their localization in the parasite is compatible with a mechanism of excretion or secretion...

journal_title：Experimental parasitology

authors： Charif H,Darcy F,Torpier G,Cesbron-Delauw MF,Capron A

更新日期：1990-07-01 00:00:00

abstract：:With the use of [3H]heparin, we recently demonstrated that Leishmania donovani promastigotes express a cell-surface receptor that is specific for the glycosaminoglycan heparin (Mukhopadhyay et al. 1989, The Biochemical Journal, 264, 517-525.). Treatment of the parasite with trypsin abolishes 75-90% of this [3H]heparin...

journal_title：Experimental parasitology

authors： Butcher BA,Shome K,Estes LW,Choay J,Petitou M,Sie P,Glew RH

更新日期：1990-07-01 00:00:00

abstract：:Evidence is given for the existence of a parasite-specific glucose-6-phosphate dehydrogenase (G6PD) in Plasmodium berghei by characterization of its kinetic and electrophoretic properties. From infected rat erythrocytes the parasites were isolated, washed, and lysed. G6PD was purified by affinity chromatography with 2...

journal_title：Experimental parasitology

authors： Buckwitz D,Jacobasch G,Kuckelkorn U,Plonka A,Gerth C

更新日期：1990-04-01 00:00:00

abstract：:Kinetoplastid organisms possess a unique organelle, the glycosome, which compartmentalizes the Embden-Meyerhof segment of glycolysis and several other metabolic pathways. In Trypanosoma brucei many of the enzyme activities localized to the glycosome are stage regulated. Two-dimensional gel analysis was used to examine...

journal_title：Experimental parasitology

authors： Parsons M,Nielsen B

更新日期：1990-04-01 00:00:00

abstract：:A limiting dilution assay (LDA) was developed for the quantification of Trypanosoma cruzi in the heart and blood of infected mice. Three groups of swiss mice were injected ip with "CL", "Colombiana," and "Y" strains. At 1-day intervals after infection, blood and the heart were removed. Serial blood dilutions in LIT me...

journal_title：Experimental parasitology

authors： Nunes MP,Coutinho SG,Louis JA,Souza WJ

更新日期：1990-02-01 00:00:00

abstract：:Rabbit antisera were raised against an 18 amino acid-long peptide that corresponds to the predicted sequence of the carboxy-terminal, nontriple helical region of the Haemonchus contortus 3A3 collagen gene. This sequence is highly conserved and diagnostic for members of the col-l collagen family, which includes the 3A3...

journal_title：Experimental parasitology

authors： Cox GN,Shamansky LM,Boisvenue RJ

更新日期：1990-02-01 00:00:00

abstract：:A drug-resistant Trypanosoma congolense strain with predetermined curative doses (CD50 and CD90) of samorin at 13.9 +/- 1.02 and 20.3 +/- 1.13 mg/kg body weight, respectively, was cyclically transmitted through tsetse flies and by syringe passages in mice in the absence of drug pressure. The changing levels of drug se...

journal_title：Experimental parasitology

authors： Nyeko JH,Golder TK,Otieno LH,Ssenyonga GS

更新日期：1989-11-01 00:00:00

abstract：:Three membrane thermotropic transitions at 8, 20, and 40 degrees C have been detected in human red blood cells (RBC) by using spin-labeled stearic acids. Red blood cells infected in vitro by Plasmodium falciparum showed the disappearance of the 8 degrees C transition and a lowering of the 40 degrees C transition to 32...

journal_title：Experimental parasitology

authors： Forte T,Roca L

更新日期：1989-10-01 00:00:00